We will develop and apply modern vibrational spectroscopic techniques,

principally time-resolved FTIR and time resolved resonance Raman to study

1. protein dynamics

2. excited state structure of porphyrins

3. vibrational relaxation

4. activation mechanism of oxygen by heme enzymes

5. electron and proton transfer through biological membranes and their coupling mechanism.

6. higher order structure of proteins and allosteric effects.

7. structure of metalloproteins and their reaction intermediates

The main techniques that will be used for these 7 labs are time resolved

FTIR and time-resolved resonance Raman, which reveal the vibrational

spectra of chromophores of large proteins . Various Lasers from UV to near

IR will be available for Raman excitation, and nanoseconds pump/probe

experiments as well as micro-to-milliseconds flow transient FTIR and Raman

will be carried out for time-resolved measurements.

One semester course